A rapid biosensor for highly sensitive protein detection based on G-quadruplex-Thioflavin T complex and terminal protection of small molecule-linked DNA
| Autor: | Jinhua Shang, Zhaohui Li, Jia Ge, Jia Gao, Dong-Mei Bai, Zhang Lin, Danyang Ji, Yalei Hu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Streptavidin
Chemistry Guanine 010401 analytical chemistry Metals and Alloys 010402 general chemistry Condensed Matter Physics G-quadruplex 01 natural sciences Small molecule Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Biochemistry Biotinylation Materials Chemistry Biophysics heterocyclic compounds Electrical and Electronic Engineering Instrumentation Biosensor DNA |
| Zdroj: | Sensors and Actuators B: Chemical. 252:1146-1152 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.07.181 |
| Popis: | Small molecule-protein interaction plays a prominent role in biological and clinical research. In this paper, based on Guanine (G)-quadruplex-thioflavin T (ThT) complex and terminal protection of small molecule-linked DNA, a simple and rapid biosensor for protein detection has been developed. This strategy was demonstrated by taking the streptavidin (SA)-biotin system as a model. In the presence of ThT, G-rich biotinylated ssDNA could transform to G-quadruplex structure and form G-quadruplex-ThT complex showing stronger fluorescence compared with free fluorogenic dye ThT. In the absence of SA, G-quadruplex was digested by exonuclease I (Exo I) to liberate ThT generating an extremely weak fluorescence signal. With the addition of SA, the specific binding between SA and biotin confirmed that G-quadruplex-ThT complex was protected against the degradation by Exo I, and the strong enhancement of the fluorescence intensity was observed. A linear correlation equation was obtained for SA from 0.05 to 2500 ng/mL with a low detection limit of 0.02 ng/mL. In addition, satisfactory results were obtained from detection of SA in 5% human serum. |
| Databáze: | OpenAIRE |
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