Gas-chromatographic analysis of poly(3-hydroxyalkanoates) in bacteria
| Autor: | Gerrit Eggink, Clare Wilkinson, Hetty van der Wal, Gern N. M. Huijberts |
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| Rok vydání: | 1994 |
| Předmět: |
Chromatography
biology Pseudomonas chemical and pharmacologic phenomena Alcaligenes eutrophus biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Pseudomonas putida Pseudomonadales bacteria lipids (amino acids peptides and proteins) Gas chromatography Alcaligenes Bacteria Pseudomonadaceae |
| Zdroj: | Biotechnology Techniques. 8:187-192 |
| ISSN: | 1573-6784 0951-208X |
| DOI: | 10.1007/bf00161588 |
| Popis: | The accuracy and reproducibility of the gas-chromatographic method for the analysis of PHB and PHA in whole cells of Alcaligenes eutrophus H16 and Pseudomonas putida KT2442 were determined. It was found that for analysis of PHA the methanolysis time in the assay had to be increased to 4 h. Accuracy of the PHB and PHA assay were 0.018 mg and 0.304 mg respectively, based on estimation of the measurement error. |
| Databáze: | OpenAIRE |
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