Analysis ofcis-platinum using a microcolumn with a β-cyclodextrin stationary phase and electrochemical detection
| Autor: | Petr Dolezel, Eva Smolková-Keulemansová, Dana Kourilova, M. Krejčí |
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| Rok vydání: | 1990 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification Body fluid Chromatography Cyclodextrin Silica gel Mechanical Engineering Filtration and Separation Cis-platinum female genital diseases and pregnancy complications chemistry.chemical_compound chemistry Stationary phase Phase (matter) Mole Acetonitrile |
| Zdroj: | Journal of Microcolumn Separations. 2:241-244 |
| ISSN: | 1520-667X 1040-7685 |
| DOI: | 10.1002/mcs.1220020507 |
| Popis: | A simple method for determining chemotherapeutically active cis-platinum using a β-cyclodextrin stationary phase on a silica gel carrier (β-CD-sil) is described. Retention of CDDP is considerably influenced by the content of acetonitrile in the mobile phase. With an electrochemical detector in oxidation mode, the minimal detectable amount of CDDP was determined to be 1.6 · 10−11 mol, and the minimal detectable concentration in the sample was 5 ppm (5 mg/mL). The method was used to determine CDDP concentration in the samples of body fluid (ascites). |
| Databáze: | OpenAIRE |
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