| Autor: |
Cristian David Peña Martinez, Mahdi Zeraati, Romain Rouet, Ohan Mazigi, Brian Gloss, Chia-Ling Chan, Tracy M. Bryan, Nicole M. Smith, Marcel E. Dinger, Sarah Kummerfeld, Daniel Christ |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.04.14.488274 |
| Popis: |
DNA i-motif structures are formed in the nucleus of human cells and are believed to provide critical genomic regulation. While the existence of i-motif structures in human cells has been demonstrated by immunofluorescent staining and by characterisation of select model genes, the abundance and distribution of such structures in the human genome has remained unclear. Here we utilize high affinity i-motif immunoprecipitation followed by sequencing to map i-motifs in human genomic DNA. Validated by biolayer interferometry and circular dichroism spectroscopy, our approach identified over 650,000 i-motif structures in human genomic DNA. The i-motif structures are widely distributed throughout the human genome and are common among highly expressed genes and in genes upregulated in G0/G1 cell cycle phase. Our findings provide experimental evidence for the widespread formation of i-motif structures in human genomic DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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