Feedback Control of the Arachidonate Cascade in Osteoblastic Cells by 15-deoxy-Δ12,14-Prostaglandin J2
| Autor: | Masahide Hamaguchi, Hidetaka Ishino, Masatoshi Kadoya, Tatsuya Hojo, Aihiro Yamamoto, Daisaku Tokunaga, Toshikazu Yoshikawa, Makoto Wada, Rikio Yoshimura, Masahide Matsuyama, Masataka Kohno, Yutaka Kawahito, Yasunori Tsubouchi |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Nutrition and Dietetics biology business.industry medicine.medical_treatment Clinical Biochemistry Medicine (miscellaneous) Troglitazone Interleukin Osteoblast Bone resorption chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Adipocyte medicine biology.protein lipids (amino acids peptides and proteins) Cyclooxygenase business Receptor Prostaglandin E medicine.drug |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition. 42:64-69 |
| ISSN: | 1880-5086 0912-0009 |
| DOI: | 10.3164/jcbn.2008011 |
| Popis: | 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) and an anti-diabetic thiazolidinedione, troglitazone (TRO) are peroxisome proliferator-activated receptor (PPAR)-gamma ligands, which regulate immuno-inflammatory reactions as well as adipocyte differentiation. We previously reported that 15d-PGJ(2) can suppress interleukin (IL)-1beta-induced prostaglandin E(2) (PGE(2)) synthesis in synoviocytes of rheumatoid arthritis (RA). IL-1 also stimulates PGE(2) synthesis in osteoblasts by regulation of cyclooxygenase (COX)-2 and regulates osteoclastic bone resorption in various diseases such as RA and osteoporosis. In this study, we investigated the feedback mechanism of the arachidonate cascade in mouse osteoblastic cells, MC3T3-E1 cells, which differentiate into mature osteoblasts. Treatment with 15d-PGJ(2) led to a significant increase in IL-1alpha-induced COX-2 expression and PGE(2) production in a dose dependent manner. The effect of 15d-PGJ(2) was stronger than that of TRO. However, it did not affect the expression of COX-1. In addition, cell viability of MC3T3-E1 cells was not changed in the condition we established. This means that 15d-PGJ(2) exerts a positive feedback regulation of the arachidonate cascade of PGE(2) in osteoblastic cells. These results may provide important information about the pathogenesis and treatment of bone resorption in a variety of diseases such as RA and osteoporosis. |
| Databáze: | OpenAIRE |
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