The Effect of Oncolytic Reovirus P-92 on Dendritic Cell Maturation and Generation of Tumor-Specific Lymphocytes in vitro
Autor: | Inna Arnoldovna Novikova, Elena S. Bondarenko, Elena P. Kolpakova, Anastasia O. Sitkovskaya, Sergey A. Kolpakov, Irina V. Mezhevova, E. Yu. Zlatnik |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology medicine.medical_treatment Cell Cell Biology Dendritic cell biology.organism_classification Tumor antigen Oncolytic virus HeLa 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Immune system Cancer immunotherapy Antigen Cancer research medicine |
Zdroj: | Cell and Tissue Biology. 13:434-438 |
ISSN: | 1990-5203 1990-519X |
Popis: | Utilization of oncolytic viruses (OVs) is a promising approach to inhibit the tumor growth. OVs exhibit both direct antitumor and indirect immunostimulatory effects. It is known that the recognition of tumor antigen by cells of the immune system is disturbed at cancer pathologies. Stimulation of dendritic cells (DCs) with tumor antigens together with viruses may enhance the generation of antigen-specific lymphocytes against tumor cells, which in turn can be an effective tool for antitumor cell technologies. The aim of this study was to clarify the possibility of using reovirus P-92 for the generation of tumor-specific T-lymphocytes in vitro. We found that the differentiation of DCs in the presence of reovirus P-92 increased the yield of mature DCs. In addition, DCs with loaded HeLa antigens after cocultivation with lymphocytes and HeLa cells did not demonstrate an active cytolytic activity. Only a cytostatic effect was observed, unlike in the case of samples with the added reovirus P-92. Thus, the antigen, which is HeLa cells cultured with reovirus P-92 under the conditions we described, can be proposed as an effective experimental product for the cellular cancer immunotherapy. |
Databáze: | OpenAIRE |
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