Novel ultrastructural observations of pea (Pisum sativum) root nodule cells by high-pressure freezing and propane-jet freezing techniques

Autor:	William Newcomb, A. L. Davidson
Rok vydání:	2000
Předmět:	Root nodule biology Endoplasmic reticulum food and beverages Cell Biology Plant Science General Medicine medicine.disease_cause biology.organism_classification Rhizobium leguminosarum Pisum Peribacteroid membrane Sativum Botany medicine Biophysics Ultrastructure Ethanesulfonic acid
Zdroj:	Protoplasma. 213:55-66
ISSN:	1615-6102 0033-183X
DOI:	10.1007/bf01280505
Popis:	Pea (Pisum sativum) root nodule cells infected by the diazotrophRhizobium leguminosarum have been well characterized by chemical fixation techniques. Propane-jet freezing and high pressure freezing were used in this study to compare rapidly frozen and chemically fixed pea root nodule cells. Cells that had been incubated in 2-(N-morpholino)ethanesulfonic acid buffer and frozen with the propane-jet freezer were better preserved than cells that had been chemically fixed or frozen with the high-pressure freezer. Rapidly frozen infected nodule cells showed that the rough endoplasmic reticulum had a high frequency of associations with the peribacteroid membrane and the infection thread. The peribacteroid space also varied in size depending on the method of preservation; however, it was most reduced in size and devoid of inclusions in the propane-jet frozen tissue. The biological significance of these observations is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5b114ce260fe62b3935774515e6f3db4 https://doi.org/10.1007/bf01280505 Zobrazit plný text záznamu Full text from SpringerLink