Evaluation Of The Efficacy Of Fluorescent Staining And Chicago Sky Blue Staining As Methods For Diagnosis Of Dermatophytosis In Hair And Nails
| Autor: | Reham Hassan, Basma Mourad, Mayada A. Ismail, Mina Msseha, Soha Abdalla Hawwam |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
food.ingredient integumentary system business.industry Dermatology Calcofluor-white medicine.disease Stain Staining 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine food medicine.anatomical_structure 030220 oncology & carcinogenesis Nail (anatomy) medicine Fluorescent staining Agar Tinea capitis business Calcofluor White Stain |
| Zdroj: | Clinical, Cosmetic and Investigational Dermatology. 12:751-758 |
| ISSN: | 1178-7015 |
| Popis: | Purpose Dermatophytes are fungi that cause infections affecting hair, nail, and skin; in nails they cause onychomycosis, while in hair they lead to tinea capitis. Detection of dermatophytes using traditional methods, including potassium hydroxide (KOH) and culture on agar-based media leads to high rates of false-negative results. Here, we investigated more accurate diagnostic techniques, including Chicago sky blue staining and Calcofluor white fluorescent staining and compared them with traditional KOH and culture methods for the diagnosis of fungi causing onychomycosis and tinea capitis. Patients and methods This study was conducted using samples from 50 patients with dermatophytosis of the hair or nail. Samples were subjected to all the following laboratory investigations: KOH wet mount, culture on Sabouraud's dextrose agar, Chicago sky blue staining, and Calcofluor white staining. The results of the new diagnostic techniques were compared with those of the traditional methods. Results Calcofluor white stain and Chicago sky blue stain for dermatophytosis of hair and nail are more specific and sensitive as compared to traditional diagnostic methods. KOH wet mount is simple, rapid, and inexpensive test but lacks color contrast and gave more false positive (artifacts) and false-negative results as compared to these new stain methods. Conclusion Chicago sky blue and Calcofluor white staining are excellent methods for diagnosis of fungal infections, including those that cannot be confirmed using conventional methods. |
| Databáze: | OpenAIRE |
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