Characteristics of the response of natural and recombinant luminescent microorganisms in the presence of Fe2+ ions

Autor:	Dmitrij Gennad'evich Deryabin, Ilshat Karimov, Dmitry Deryabin
Rok vydání:	2010
Předmět:	chemistry.chemical_classification Strain (chemistry) Operon Microorganism Photobacterium phosphoreum Fatty acid Biology biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Divalent Microbiology law.invention chemistry law Recombinant DNA Bioluminescence
Zdroj:	Applied Biochemistry and Microbiology. 46:28-32
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683810010047
Popis:	It was found that divalent iron ions have alternative effects on the bioluminescence of the natural marine microorganism Photobacterium phosphoreum and the recombinant Escherichia coli strain with a cloned lux operon of P. leiognathi. In the presence of 0.25–5.0 mM FeSO4, the bioluminescence intensity of the former and the latter increased and decreased, respectively. To establish the causes of these differences, we studied the characteristics of the fatty acid composition of the compared microorganisms. The fatty acid profile of E. coli was characterized by a high proportion of unsaturated 11-octadecenoic (vaccenic) acid. A study of this acid in a cell-free enzyme system used for bioluminescence generation showed that it is a potent inhibitor of bacterial bioluminescence. It was found that such effects are enhanced if 11-octadecenoid acid is preincubated with Fe2+.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5af7fd533e9e0343876ef004b2458021 https://doi.org/10.1134/s0003683810010047 Zobrazit plný text záznamu