| Autor: |
Maykon Paiva, Fabrício Paula-Elias, Luciana Pereira, Solange Carreiro, Erika Vieira-Almeida, Ezequiel Silva, Giancarlo Dias, Michelle Xavier, Sergio Morales, Rafael Perna, Alex Almeida |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of the Brazilian Chemical Society. |
| ISSN: |
0103-5053 |
| DOI: |
10.21577/0103-5053.20230067 |
| Popis: |
The operational conditions of β-galactosidase encapsulated in pectin-alginate hydrogel were investigated as well as its catalytic activity in the hydrolysis of lactose from whey and whole milk. β-Galactosidase encapsulated in pectin-alginate hydrogel showed the best results to yields and diffusional effect and pH range. Thermostability showed considerable income gain for half-life (1.7-fold), activation energy of denaturation (31.6-fold), activation of denaturation (217.3-fold at 40 ºC; 266.8-fold at 50 ºC; 345.6-fold at 60 ºC), and entropy of activation of denaturation (−42.0 J mol-1 K-1). Reusability of encapsulated β-galactosidase was observed in 8-cycles for the milk whey lactose hydrolysis (51.9%) and 7-cycles for whole milk (55.6%), and the lactose hydrolysis was observed in 13% in milk whey and 10.3% in whole milk after 10 cycles. These results revealed an industrial application potential of β-galactosidase encapsulated in pectin-alginate hydrogel for lactose hydrolysis of milk whey and whole milk processes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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