Fate of pBR322 DNA in a wastewater matrix
| Autor: | D R Orvos, A L Worthey, J F Kane |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
business.industry
Bioengineering Biology Applied Microbiology and Biotechnology Biotechnology law.invention Matrix (chemical analysis) Transformation (genetics) chemistry.chemical_compound Activated sludge Plasmid Wastewater Biochemistry chemistry law Recombinant DNA business Polymerase chain reaction DNA |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 22:164-166 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1038/sj.jim.2900629 |
| Popis: | Recombinant organisms used in biopharmaceutical production processes are destroyed prior to environmental release into a private or municipal wastewater treatment system. However, concern over the fate of recombinant DNA used in these processes may adversely affect product regulatory approval. This study examined the fate of DNA from the plasmid pBR322 in an activated sludge-derived matrix. DNA suitable for PCR amplification was extracted from the activated sludge matrix and a 1042-bp fragment from pBR322 rapidly decreased in concentration from 0 to 2 h after it was spiked into the activated sludge matrix at an initial DNA concentration of 25 ng ml−1. While some evidence of the 1042-bp fragment was observed at 4 h, no evidence of amplified DNA was observed at 6 h. Plasmid DNA in buffer that served as a positive control exhibited no significant reduction in concentration over time. The intensity of each DNA band over the first 4 h was analyzed. A linear regression of the natural log transformation of these results yielded a mean first-order rate constant of 3.55 h−1 and half-life of 0.2 h. This study demonstrated that recombinant DNA released from industrial processes into wastewater treatment systems should be rapidly degraded. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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