Androgen-Dependent Differences in the Amounts of CYP mRNAs in the Pig Kidney
| Autor: | Misaki Kojima, Masakuni Degawa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Gene isoform Testosterone propionate Kidney medicine.medical_specialty medicine.drug_class CYP1A2 Pharmaceutical Science General Medicine Biology urologic and male genital diseases Androgen Breed chemistry.chemical_compound medicine.anatomical_structure Castration Endocrinology chemistry Internal medicine medicine Testosterone |
| Zdroj: | Biological and Pharmaceutical Bulletin. 44:1120-1128 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | We previously reported androgen-dependent sex and breed differences in the amounts of mRNAs of CYP isoforms in the pig liver. To clarify whether there are such sex and breed differences in the kidney, we examined the amounts of several CYP mRNAs in the kidney using both sexes of 5-month-old Landrace, Meishan and/or their crossbred F1 (LM and ML) pigs. Significant sex differences in the amounts of several CYP mRNAs were found: male female for CYP4A24/25 in all the breeds. Sex differences in the amount of CYP2B22 mRNA (male female) were also observed in all the breeds except Landrace pigs. Furthermore, a significant sex difference (male < female) in CYP3A46 mRNA was only found in LM and ML pigs. No significant sex differences were found in either Landrace or Meishan pigs for CYP1A1, CYP1A2 and CYP4B1 mRNAs. The amounts of CYP2C33 and CYP4A24/25 mRNAs in males were higher in Meishan pigs than in Landrace pigs. Additional experiments using pigs treated by castration and/or testosterone propionate indicated that sex and breed differences in the amounts of those CYP mRNAs were, at least in part, dependent on the levels of serum testosterone. Furthermore, the effects of androgen on the amounts of CYP mRNAs in the kidney did not necessarily correlate with those in the liver, suggesting that there is a tissue-selective factor responsible for the androgen-related expression of CYP genes. |
| Databáze: | OpenAIRE |
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