| Popis: |
We describe here our work toward automation of the analysis of postmortem samples or buccal swabs and brushes, using FTA® cards. First we manually spot the samples on the FTA®. Then we perform their analysis, according to our high-throughput process. The genotypes of spotted samples are compared to those obtained from the original samples manually extracted, to estimate the reproducibility. Then, the impact on the genotypes' quality of the amplification of 1, 2 or 3 FTA® punches in the same well is assessed. Our results show that: 1) The quality of the profile from a FTA is positively correlated to the quantity of DNA obtained by manually treatment of the sample. 2) No sample leads systematically to acceptable results. 3) In few cases, the addition of more than one punch in the same reaction can ameliorate the profiles. However, the improvement is not correlated to the quantity of DNA in the original samples. In conclusion, this technique is convenient for DNA typing as it offers all the advantages of the subsequent automated treatment. Yet, some cases may need multiple analysis to obtain a reliable profile. |
Full Text from ScienceDirect