Raman and fluorescence spectroscopic evaluation of NIR laser-welded human and porcine aorta tissues
| Autor: | Alvin Katz, Tapan K. Gayen, Rabindra K. Halder, Alexandra Alimova, Glenn Minko, Steven A. McCormick, Howard E. Savage, Cheng-hui Liu, Robert R. Alfano |
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| Rok vydání: | 2003 |
| Předmět: |
Absorption (pharmacology)
Aorta Materials science business.industry Near-infrared spectroscopy technology industry and agriculture Welding respiratory system Laser Fluorescence law.invention symbols.namesake Optics law medicine.artery Biophysics medicine symbols Luminescence business Raman spectroscopy |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.476390 |
| Popis: | Laser tissue welding involves the partial denaturing and renaturing of the collagen triple helical structure. Although themechanisms of laser tissue welding are not well understood, water in tissues plays an important role in the process. Highquality welding of human and porcine aorta tissue have been achieved using NIR lasers tuned to the water absorption bandaround 1450 nm. Fluorescence and Raman spectra from welded and non-welded regions are compared for ex vivo humanand porcine aorta tissues. The fluorescence from the outer surface of welded aorta was substantially weaker than thefluorescence from the non-welded region. The Raman spectra from the welded and non-welded tissue regions appearedsimilar in the energies of the observed vibrational levels but the intensity of the fluorescence wing was considerably greaterfrom the outer surface of the welded region as compared to the non-welded region. For the outer surface of the aorta, theemission intensity from the welded region was larger than for the non-welded region.Key Words: Tissue welding, collagen, fluorescence, Raman spectroscopy |
| Databáze: | OpenAIRE |
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