Linear-after-the-exponential (LATE)-PCR: Improved asymmetric PCR for quantitative DNA-analysis

Autor:	M. Schürenkamp, Ursula Sibbing, K. Schulze Johann, Marielle Vennemann, C. Lischka, Heidi Pfeiffer
Rok vydání:	2015
Předmět:	chemistry.chemical_compound Real-time polymerase chain reaction chemistry Linear amplification Genetics Asymmetric PCR Primer (molecular biology) Biology Molecular biology DNA Pathology and Forensic Medicine Exponential function
Zdroj:	Forensic Science International: Genetics Supplement Series. 5:e659-e661
ISSN:	1875-1768
DOI:	10.1016/j.fsigss.2015.09.246
Popis:	Linear-after-the-exponential (LATE)-PCR describes a novel approach to asymmetric PCR which uses adjusted melting temperatures of the limited primer to increase PCR efficiency. In this proof-of-principle study we show that linear amplification is possible over a wide range of amplification cycles. The curve characteristics of the real time PCR show that the plateau effect of conventional PCR can be avoided by this innovative technique.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5ac1e03a36beb4bf9d13356bf5babf44 https://doi.org/10.1016/j.fsigss.2015.09.246 Zobrazit plný text záznamu Full Text from ScienceDirect