Frequency and antibiogram pattern of gram positive cocci in catheter related blood stream infections (CRBSI) in a tertiary care hospital, Tamilnadu
| Autor: | Akila Akila, S. Senthamarai, S Sivasankari, V.M. Somasunder, Kumuthavathi Kumuthavathi, Rajesh. J.G, C. Anitha |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
biology
medicine.diagnostic_test business.industry Gram-positive bacteria medicine.disease_cause biology.organism_classification Microbiology chemistry.chemical_compound chemistry Staphylococcus aureus Ampicillin Linezolid medicine Vancomycin Blood culture Coagulase business Gram-Positive Cocci medicine.drug |
| Zdroj: | Tropical Journal of Pathology and Microbiology. 3:102-106 |
| ISSN: | 2456-1487 2456-9887 |
| DOI: | 10.17511/jopm.2017.i02.04 |
| Popis: | Introduction: Blood stream infection associated with catheter is life threatening. Various bacterial agents are responsible for this and Coagulase Negative Staphylococci (CoNS) is now becoming one of the major agent in Catheter Related Blood Stream infection (CRBSI). Objectives: To know the bacterial isolates and the frequency of the CoNS & its antibiotic resistance patterns of in Catheter Related Blood Stream infection (CRBSI). Methods: Catheter tips (5 cm tip) were collected and 5 ml of peripheral blood sample was collected with proper aseptic precautions. Catheter tips were processed using Maki DG et al procedure. Blood was inoculated into the BACTEC blood culture bottle (BacT/ALERT 3D automated blood culture system –BioMerieux). All the gram positive bacteria were further processed for standard biochemical tests. All these isolates were subjected to antibiotic susceptibility testing (Hi-Media discs) by Kirby Bauer disc diffusion test according to CLSI guidelines. Results: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase Negative Staphylococci (CoNS), were the predominant isolate in this study. Highest resistance was observed with ampicillin, penicillin, amoxyclav, erythromycin and tetracycline. All the gram positive isolates were 100% sensitive to Vancomycin and Linezolid. Conclusion: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase negative staphylococci (CONS) which account 18.98%, followed by 8.86%, Staphylococcus aureus, 5.06% Enterococci and 1.89% were Micrococci. All our isolates were sensitive to Vancomycin and linezolid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|