Characterization and comparison of rat monosodium iodoacetate and medial meniscal tear models of osteoarthritic pain
| Autor: | Arthur L. Nikkel, Stella Markosyan, Michael F. Jarvis, Katharine L. Chu, Jill-Desiree Brederson, Robert S. Bitner, Rebecca M. Edelmayer, Steve McGaraughty, Jun Xu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty business.industry Chronic pain Arthritis Stimulation Osteoarthritis medicine.disease Spinal cord Somatosensory system 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine Orthopedics and Sports Medicine business Prefrontal cortex 030217 neurology & neurosurgery Sensitization |
| Zdroj: | Journal of Orthopaedic Research®. 36:2109-2117 |
| ISSN: | 0736-0266 |
| DOI: | 10.1002/jor.23869 |
| Popis: | Osteoarthritis (OA) is a degenerative form of arthritis that can result in loss of joint function and chronic pain. The pathological pain state that develops with OA disease involves plastic changes in the peripheral and central nervous systems, however, the cellular mechanisms underlying OA are not fully understood. We characterized the medial meniscal tear (MMT) surgical model and the intra-articular injection of monosodium iodoacetate (MIA) chemical model of OA in rats. Both models produced histological changes in the knee joint and associated bones consistent with OA pathology. Both models also increased p38 activation in the L3, but not L4 dorsal root ganglia (DRG), increased tyrosine hydroxylase immunostaining in the L3 DRG indicating sympathetic sprouting, and increased phosphorylated (p)CREB in thalamic neurons. In MIA-OA, but not MMT-OA rats, p38 and pERK were increased in the spinal cord, and pCREB was enhanced in the prefrontal cortex. Using in vivo electrophysiology, elevated spontaneous activity and increased responsiveness of wide dynamic range neurons to stimulation of the knee was found in both models. However, a more widespread sensitization was observed in the MIA-OA rats as neurons with paw receptive fields spontaneously fired at a greater rate in MIA-OA than MMT-OA rats. Taken together, the MIA and MMT models of OA share several common features associated with histopathology and sensitization of primary somatosensory pathways, but, observed differences between the models highlights unique consequences of the related specific injuries, and these differences should be considered when choosing an OA model and when interpreting data outcomes. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. |
| Databáze: | OpenAIRE |
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