Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L

Autor:	Daisuke Igawa, Ram Savan, Masahiro Sakai
Rok vydání:	2003
Předmět:	Genetics Untranslated region Biology biology.organism_classification Biochemistry Molecular biology Open reading frame Exon Common carp Complementary DNA sense organs Carp Peptide sequence Gene
Zdroj:	European Journal of Biochemistry. 270:4647-4654
ISSN:	1432-1033 0014-2956
Popis:	Interleukin (IL)-10 was cloned from the common carp (Cyprinus carpio L.) using IL-10 primers from carp head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp sequence containing a 55 bp 5' untranslated region and a 498 bp 3' untranslated region. An open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids. The signature motif of IL-10 is conserved in carp sequence. A 2083 bp genomic sequence of carp IL-10 was found to contain five exons interrupted by four introns. With the exception of much more compact introns, the genomic structure was similar to that of mammalian IL-10. By homology, phylogeny and genomic analyses, the carp gene cloned was designated as IL-10. Carp IL-10 was expressed in head, kidney, liver, spleen and intestine during the resting phase. The gene was also expressed in head kidney and liver following in vitro stimulation with lipopolysaccharide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5a9512e30cc7c7517b0f068ff280ebba https://doi.org/10.1046/j.1432-1033.2003.03854.x Zobrazit plný text záznamu Plný text ve formátu PDF