| Autor: |
Corine Ferrua, Jean-Pierre Girard, Danielle Pesando |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Aquatic Toxicology. 38:225-239 |
| ISSN: |
0166-445X |
| DOI: |
10.1016/s0166-445x(96)00845-4 |
| Popis: |
Tributyltin (TBT) is one of the widespread organotins in the marine environment: we have investigated its cellular targets in the eggs of the marine invertebrate sea urchin Paracentrotus lividus . TBT was used at concentrations ranging from 10 −9 to 10 −4 M. The lipophilic character of TBT makes that the magnitude of the effects varied according to the concentration of egg used. Concentrations of TBT being without effect on egg fertilisation inhibited first and second cleavage in a dose-dependent manner. Concentrations of TBT which block egg cleavage did not alter egg membrane permeabilities to Ca 2+ and Na + . TBT inhibited intracellular ATP-driven sequestration of Ca 2+ into reticular compartment but did not provoke Ca 2+ leakage from these compartment. Blockade of eggs cleavage by TBT is accompanied by an inhibition of protein synthesis ( 35 S-methionine incorporation) and DNA synthesis ( 3 H-thymidine incorporation). Protein phosphorylation ( 32 P incorporation) and histone H1 kinase activity were inhibited when cell cycling was arrested by organotin. Results are discussed in terms of concentration of TBT per egg protein in order to compare the particular sensitivity of the numerous cellular process affected by TBT. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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