Optimization of Heavy Metal Removal by Sulfate Reducing Bacteria in a High Concentration Zn-fed Fixed Bed Bioreactor Using Plackett Burman Design Experiments

Autor: He Shang, Qiyuan Gu, Cui Xinglan, Jiankang Wen, Xingyu Liu
Rok vydání: 2018
Předmět:
Zdroj: IOP Conference Series: Earth and Environmental Science. 182:012012
ISSN: 1755-1315
DOI: 10.1088/1755-1315/182/1/012012
Popis: This study evaluated the combined effect of six processing factors on Zn, Cd removal and sulfate reducing by sulfate reducing bacteria in a high concentration Zn-fed fixed bed bioreactor. Statistically valid Plackett Burman design of experiments was employed to carry out this study. The results obtained showed a high removal of Zn (99.63%), Cd (99.73%) at a maximum influent Zn concentration of 320 mg/L. Analysis of variance (ANOVA) of Zn and Cd removal revealed that the effect due to glycerol dosage and reflux ratio were highly significant (P value < 0.05). To establish the role of sulfate reducing bacteria (SRB) in the metal removal process, the characteristics of microbial community in fixed bed bioreactor was analyzed by High-throughput Sequencing during the steady operational process. The results obtained shows that the percentage of the dominant sulfate reducing bacteria in the sludge, such as Desulfovibrio, could reach 45.7%. Qualitative EDS analysis of the precipitate was performed. The results revealed that the precipitates as ZnS were confirmed by the EDS spectrum with strong peaks of zinc and sulfur.
Databáze: OpenAIRE