Identification of Host Proteins Packaged in H5N1 Avian Influenza Virus Particles Propagated in Embryonated Chicken Eggs by Mass Spectrometry

Autor:	Ning Liu, Yue Zhou, Zhong-Yi Cong, Qing-Wei Zhou, Wan-Chun Sun, Zhi-Wu Wang
Rok vydání:	2012
Předmět:	chemistry.chemical_compound Phosphoglycerate kinase chemistry Biochemistry Embryonated Sodium dodecyl sulfate Cytoskeleton Proteomics Polyacrylamide gel electrophoresis Molecular biology Virus Pyruvate kinase Analytical Chemistry
Zdroj:	Chinese Journal of Analytical Chemistry. 40:376-380
ISSN:	1872-2040
DOI:	10.1016/s1872-2040(11)60537-9
Popis:	In this study, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) coupled with mass spectrometry was used to perform a comprehensive proteomics analysis of the purified influenza virus particles propagated from embryonated chicken eggs. In addition to the virus-encoded proteins, 12 proteins originated from embryonated chicken eggs were identified in the purified virus particles, such as annexin A2, peptidyl-prolyl cis-trans isomerase B, peroxiredoxin-1, phosphoglycerate kinase, and pyruvate kinase muscle isoenzyme. Two kinds of cytoskeletal proteins, tubulin b-3 and actin, were also detected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5a66cd0a0c7b85868d2fbd77f880ca31 https://doi.org/10.1016/s1872-2040(11)60537-9 Zobrazit plný text záznamu Full Text from ScienceDirect