| Popis: |
A colorimetric method developed for Thimet eliminates many of the objectionable features of other methods currently in use. The procedure is fairly sensitive, requires little cleanup, and, except for minor alteration of standard laboratory glassware, requires no specialized equipment. Thimet and its metabolites are completely extracted from the plant material by chloroform, an aliquot is passed through a Florisil column, and the chloroform is evaporated. The residue containing Thimet and its oxidative products is picked up in a hexane-acetonewater system. The oxidized metabolites are removed by partitioning into the acetonewater phase. The hexane phase, containing Thimet, is evaporated to dryness and hydrolyzed with 48% hydrobromic acid. The hydrogen sulfide thus generated is determined colorimetrically as methylene blue. |
