Genomic structure, polymorphism and expression of ACCN1 and ACCN3 genes in the horse
| Autor: | Gérard Guérin, Mathieu Diribarne, A. Ducasse, Anne Vaiman, A.‐S. Fraud, Xavier Mata |
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| Rok vydání: | 2010 |
| Předmět: |
Genetics
Gene isoform 0303 health sciences Alternative splicing General Medicine Biology Molecular biology 03 medical and health sciences Exon Open reading frame 0302 clinical medicine Gene expression Gene family Coding region Animal Science and Zoology Gene 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Animal Genetics. 41:138-144 |
| ISSN: | 0268-9146 |
| DOI: | 10.1111/j.1365-2052.2010.02123.x |
| Popis: | A category of cation gate proteins was shown to be present in sensory neurons and act as receptors of protons present in tissues such as muscles. The Amiloride-sensitive Cation Channel, Neuronal (ACCN) gene family is known to play a role in the transmission of pain through specialized pH sensitive neurons. Muscles from horses submitted to strenuous exercises produce lactic acid, which may induce variable pain through ACCN differential properties. The sequences of the equine cDNAs were determined to be 2.6 kb in length with an open reading frame of 1539 bp for ACCN1 and 2.1 kb in length with an open reading frame of 1602 bp for ACCN3. The ACCN1 gene is 990 kb long and contains 10 exons, and the ACCN3 gene is 4.2 kb long and contains 11 exons. The equine ACCN1 and ACCN3 genes have an ubiquitous expression but ACCN1 is more highly expressed in the spinal cord. We identified one alternative ACCN3 splicing variant present in various equine tissues. These mRNA variants may encode two different protein isoforms 533 and 509 amino acids long. Ten single nucleotide polymorphisms (SNPs) were detected for ACCN1; five in the coding and five in the non-coding region, with no amino acid change, while the three SNPs identified in the coding region of the ACCN3 gene introduce amino acid changes. The equine in silico promoter sequence reveals a structure similar to those of other mammalian species, especially for the ACCN1 gene. |
| Databáze: | OpenAIRE |
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