Single-chain nanoparticles containing sequence-defined segments: using primary structure control to promote secondary and tertiary structures in synthetic protein mimics
| Autor: | Ashley M. Hanlon, Jacob J. Lessard, Kyle J. Rodriguez, J. P. Mancinelli, E. K. Reville, Erik B. Berda, Justin P. Cole |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Chemistry Stereochemistry Isocyanide Organic Chemistry Nanoparticle Bioengineering Sequence (biology) 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Combinatorial chemistry Protein tertiary structure 0104 chemical sciences Folding (chemistry) chemistry.chemical_compound Covalent bond Proton NMR Non-covalent interactions 0210 nano-technology |
| Zdroj: | Polymer Chemistry. 8:5829-5835 |
| ISSN: | 1759-9962 1759-9954 |
| Popis: | We investigated intra-chain isocyanide-based multicomponent reactions to synthesize single-chain nanoparticles (SCNP) containing sequence-defined segments at each junction point in order to create materials featuring multiple protein-inspired elements. Upon intramolecular cross-linking, nanoparticle formation ensues, affording materials with well-defined structural elements situated in a disordered tertiary structure. The resulting nanostructures were characterized using 1H NMR, DOSY NMR, and size-exclusion chromatography. While covalent cross-linking was the intended and predominant mode of SCNP formation, we found that secondary, noncovalent interactions contributed significantly to nanoparticle folding more akin to natural materials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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