Evidence Supporting the Recognition of Cole latent virus as a Distinct Carlavirus

Autor:	M. L. P. N. Targon, P. Belintani, M. A. Machado, José O. Gaspar
Rok vydání:	2002
Předmět:	Genetics animal structures biology Physiology Nucleic acid sequence Potato virus M RNA Plant Science biology.organism_classification Virology Virus Latent Virus Carlavirus Complementary DNA embryonic structures Agronomy and Crop Science Subgenomic mRNA
Zdroj:	Journal of Phytopathology. 150:330-333
ISSN:	1439-0434 0931-1785
DOI:	10.1046/j.1439-0434.2002.00757.x
Popis:	Some molecular properties are described of Cole latent virus (CoLV), hitherto designated a tentative species of the Carlavirus genus. CoLV genomic RNA (Ribonucleic acid) of 8.3 Kb is polyadenylated. Two unencapsidated polyadenylated subgenomic RNAs (2.6 and 1.3 Kb) and three double-stranded RNAs (dsRNAs) (8.3, 2.6 and 1.3 Kbp), which are twice the size of the genomic and subgenomics ssRNAs, are produced in CoLV-infected plants; two additional dsRNAs (7.2 and 6.3 Kbp) were also detected in plant extracts. By using a Carlavirus specific primer and a CoLV cDNA, a-3'-terminus fragment of 116 bp was amplified; it had homology with the carlaviruses Potato virus M (62%), Hop latent virus (37%) and Blueberry scorch virus (36%) but no significant homology with 11 other carlaviruses. These results support the classification of CoLV as a distinct species of the Carlavirus genus.
Databáze:	OpenAIRE
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