Autor: |
Dingbo Zhou, Theophanes Solomos, Autar K. Mattoo, Hidemasa Imaseki |
Rok vydání: |
1999 |
Předmět: |
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Zdroj: |
Journal of Plant Physiology. 154:346-350 |
ISSN: |
0176-1617 |
DOI: |
10.1016/s0176-1617(99)80178-3 |
Popis: |
Summary We have studied the accumulation of acid invertase isozymes and gene transcripts in potato ( Solarium tuberosum cv. Russet Burbank) tubers stored at 10 °C or 1 °C. Northern blot analysis using a cDNA probe to vacuolar invertase showed that the invertase transcript does not accumulate in tubers stored at 10 °C. However, in tubers stored at 1 °C the invertase transcript level increases markedly within 7 days. The accumulation of acid invertase transcripts was found coincident with an increase in invertase activity, mainly the isoform with a pi value of 5.37. Southern blot analysis indicated that the vacuolar acid invertase gene is probably present in one or two copies per haploid genome. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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