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Publisher Summary Capillary electrophoresis (CE) is a rapid, high-resolution analytical technique for the separation of a wide variety of charged and uncharged species. Separations in electrophoresis are based on the differential migration rates of species through a solution or gel in the presence of an electric field. Important applications of CE include the analysis of complex biological mixtures—for example, the separation and characterization of macromolecules. The fused silica tubing does not always provide the necessary separation performance; modification of the capillary is often required. The wide acceptance of the technique and practical applications can be attributed to the advances in column technology. It is now possible to move beyond the analysis of amino acids and peptides to that of macromolecules. The chapter focuses on the advances in column technology for reducing solute adsorption, manipulating flow rate, and enhancing the efficiency and selectivity of CE. |
