Spinal Orexin-2 Receptors are Involved in Modulation of the Lateral Hypothalamic Stimulation-Induced Analgesia
Autor: | Laleh Rezaee, Amir-Mohammad Alizadeh, Sakineh Salehi, Abbas Haghparast, Soheila Fazli-Tabaei |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Carbachol Lateral hypothalamus business.industry Stimulation General Medicine Spinal cord TCS-OX2-29 Biochemistry Orexin 03 medical and health sciences Cellular and Molecular Neuroscience 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Nociception Endocrinology Internal medicine medicine Receptor business 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Neurochemical Research. 44:1152-1158 |
ISSN: | 1573-6903 0364-3190 |
Popis: | Role of the orexinergic system in pain modulation is well studied and involvement of the spinal orexin-1 receptors is well documented. In this study, we examined role of the spinal orexin-2 receptors in modulation of inflammatory pain in rat. Fifty-one adult male Wistar rats were implanted unilaterally with a guide cannula into the LH and intrathecal tubing in the lumbar space between L4 and L5. Chemical stimulation of LH by carbachol (250 nM/0.5 µL saline) induced remarkable analgesia during the two phases of formalin test and Intrathecal administration of different doses of TCS OX2 29 (10, 30 and 100 µM/ 0.5 µL DMSO) prior to LH stimulation, dose-dependently antagonized the antinociceptive effect of the LH-stimulation during the two phases of formalin test. The effect size of the TCS OX2 29 was η2 = 0.47 and η2 = 0. 87 for the early and late phases of the test, respectively. Also, intrathecal administration of TCS OX2 29 alone (without stimulation of the LH) had no significant effect on formalin induced pain-related behaviors. Our results showed that spinal orexin-2 receptors are involved in modulation of the LH-stimulation induced analgesia in a persistent inflammatory pain model. These findings may suggest spinal orexin-2 receptors in particular and the orexin system in general as a useful therapeutic target for treatment of chronic pains. |
Databáze: | OpenAIRE |
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