| Autor: | Charlotte Behr, Inès Vigan-Womas, Bartholomew D. Akanmori, Séverine Loizon, Odile Mercereau-Puijalon, Jean Christophe Barale, Delphine Jublot, Philippe Boeuf |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Regulation of gene expression
0303 health sciences Messenger RNA Immunology RNA Computational biology Biology 3. Good health Gene expression profiling 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Real-time polymerase chain reaction Immune system chemistry 030220 oncology & carcinogenesis Gene DNA 030304 developmental biology |
| Zdroj: | BMC Immunology. 6:5 |
| ISSN: | 1471-2172 |
| DOI: | 10.1186/1471-2172-6-5 |
| Popis: | Background Real-time PCR is becoming a common tool for detecting and quantifying expression profiling of selected genes. Cytokines mRNA quantification is widely used in immunological research to dissect the early steps of immune responses or pathophysiological pathways. It is also growing to be of clinical relevancy to immuno-monitoring and evaluation of the disease status of patients. The techniques currently used for "absolute quantification" of cytokine mRNA are based on a DNA standard curve and do not take into account the critical impact of RT efficiency. |
| Databáze: | OpenAIRE |
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