Role of CD38 during ontogeny of murine B cells in bone marrow (153.23)
| Autor: | Hector Romero-Ramirez, Rosana Pelayo-Camacho, Ruben Lopez-Santiago, Juan Carlos Rodriguez-Alba, Leopoldo Santos-Argumedo |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 186:153.23-153.23 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | CD38 is a 45 KD transmembrane protein, expressed by immature and mature lymphocytes. Agonistic antibodies directed to this molecule induce several biological responses: cellular proliferation, protein phosphorylation, intracellular Ca2+ mobilization, rescue from and induction of apoptosis. CD38 ligation with a mAb anti-CD38 (NIM-R5) induces apoptosis of the murine pro-B cell line (Ba/F3); this phenomenon depends on tyrosine kinases activation and the MAP kinase ERK. The expression and function of CD38 during B-cell ontogeny has been poorly analyzed in mice. We analyzed the expression of CD38 during the ontogeny of B cells in murine bone marrow. CD38 is expressed from the preproB stage. ProB, preB and immature B cells from murine bone marrow were all positive for the expression of CD38, but immature B cells had the highest fluorescence intensity, and the proB stage shows a low and high expression of CD38 that suggest two probable populations. The results suggest that CD38 may play a role during the ontogeny of murine B cells. We are analysing whether CD38 ligation on these subpopulations, induces proliferation, maturation or apoptosis. |
| Databáze: | OpenAIRE |
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