123 THE MARE MODEL TO STUDY HOW OVARIAN DYNAMICS AFFECTS PREANTRAL FOLLICLE FEATURES
| Autor: | Eduardo L. Gastal, Benner Geraldo Alves, Maria Lúcia Gambarini, K.A. Alves, S. G. S. de Tarso, Melba O. Gastal, G. D. A. Gastala, José Ricardo de Figueiredo |
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| Rok vydání: | 2016 |
| Předmět: |
endocrine system
medicine.medical_specialty education.field_of_study urogenital system Population Reproductive technology Biology Antral follicle Oogenesis Follicle Endocrinology medicine.anatomical_structure Reproductive Medicine Internal medicine Follicular phase Genetics medicine Animal Science and Zoology Folliculogenesis education Molecular Biology Corpus luteum Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 28:191 |
| ISSN: | 1031-3613 |
| Popis: | The mare has been strongly advocated by several research groups as an important comparative animal model to study antral follicular dynamics in women due to some similarities in reproductive events. More recently, the mare has also been suggested as a potential model for studies related to preantral follicles. The search for an appropriate animal model for comparative studies of preantral follicle population, density, and distribution has been a major focus of recent ovarian translational studies. The aim of the present study was to investigate the influence of reproductive phase (anestrous v. diestrous) and ovarian structures (antral follicles and corpus luteum) on the quality, class distribution, number, and density of preantral follicles, and stromal cell density. Ovarian fragments were harvested in vivo from young, healthy mares (n = 10) during both reproductive phases using a biopsy pickup method and submitted to histological analysis. All evaluations and measurements were performed by a single operator and only follicles with a visible nucleus was considered for follicle counting. Data were analysed by Kruskal-Wallis test, Wilcoxon-Mann-Whitney test, and Spearman’s correlation. A total of 142 ovarian biopsy fragments were collected (mean, 3.7 fragments per mare in each reproductive phase) and 13 462 histological sections were evaluated. Overall, 1493 preantral follicles were recorded with a mean of 10.5 ± 1.7 follicles per ovarian fragment [range, 0 to 165; mean coefficient of variation (CV) = 195%]. The mean follicle density was 2.7 follicles per cm2 (range, 0 to 39; CV = 214%) and differed (P |
| Databáze: | OpenAIRE |
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