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Bacillus species produce a large number of biological compounds against bacteria and fungi. The aim of this study was isolation and diagnosis of kinds of isolates Bacillus from different plants and determination of their efficiency as a bio-control agents against phytopathogenic bacteria like Xanthomonas campestris pv. campestris andPectobacterium carotovorum subsp. carotovorum Supernatants and suspensions containing isolates Bacillus were studied for determination of antagonistic effect. Antibacterial activity performed by disk method. Cell growth and production of bioactive compounds were followed during cultivations in a medium proposed by Kim et al. (1997) for bio-surfactant production. Then bio-surfactant production was estimated by superficialtension measurements. Among of isolates Bacillus, Bacillus thuringiensis, Bacillus cereus, Bacillus subtilis, Bacillus megaterium and Bacillus pumilus against investigated phytopathogenes showed good activity. Also the study showed highest hemolytic and antimicrobial activities, to follow up growth and production of bio-surfactant compounds. Relationship between mean diameter of zone of inhibition with 3 tested type of bacteria, kind microbial solution and type of medium were significant. If the result of this method to be similar with in vivo conditions in future, it can be optimized for neutralization of bacteria pests in plants. Key words: Bacillus species, Xanthomonas campestris, Pectobacterium carotovorum, phytopathogen. |
