In vitro T lymphocyte function in primary immunodeficiency diseases
| Autor: | Patrícia O. Viana, Liziane C. Almeida, Reinaldo Salomão, Maria Isabel de Moraes-Pinto, Celso Francisco Hernandes Granato, Beatriz Tavares Costa-Carvalho |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cellular immunity
biology medicine.diagnostic_test CD3 Biochemistry (medical) Stimulation T lymphocyte medicine.disease Flow cytometry 03 medical and health sciences 0302 clinical medicine Pediatrics Perinatology and Child Health Immunology medicine biology.protein Primary immunodeficiency Interferon gamma 030212 general & internal medicine IL-2 receptor medicine.drug |
| Zdroj: | Journal of Pediatric Biochemistry. :043-052 |
| ISSN: | 1879-5404 1879-5390 |
| DOI: | 10.1055/s-0036-1586392 |
| Popis: | Diagnosis of primary immunodeficiency diseases (PID), based on laboratory tests and assessment of T lymphocyte function, is crucial in patients who present with lymphopenia. We evaluated T lymphocyte function in healthy children and adults and in patients and with PID using flow cytometry. Whole blood cultures were stimulated with phytohemagglutinin, purified protein derivate (PPD) and candidin, followed by detection of intracellular interferon-gamma (IFN - gamma) and CD25 membrane expression on CD3+ T cells by flow cytometry. Flow cytometry results were compared with 3H-thymidine (3HTdR) lymproliferation after in vitro cell stimulation and with delayed type hypersensitivity reaction (DTH). Patients with PID had lower intracellular IFN - gamma production than healthy children and healthy adults after PHA stimulation for 18 h ( p = 0.024 and p p = 0.048 and p p p = 0.029). The DTH for PPD was not different between PID and healthy children ( p = 0.281). Intracellular IFN-gamma after PHA stimulation for 18 h and CD25 membrane expression after candidin stimulation for 72 h on CD3+ T cells were most reliable parameters that could discriminate PID patients from healthy children. Our results confirm the high variability in functional cell assays and reinforce the idea that age differences must be taken into consideration during assay evaluation. |
| Databáze: | OpenAIRE |
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