Chemical characteristics, antioxidant and anticancer potential of sulfated polysaccharides from Chlamydomonas reinhardtii
Autor: | V. L. Sirisha, Sanith Cheriyamundath, Priyanka Kamble, Manu Lopus |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Antioxidant ABTS biology DPPH medicine.medical_treatment education Chlamydomonas reinhardtii Plant Science Uronic acid Aquatic Science biology.organism_classification 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Column chromatography Sulfation chemistry Biochemistry 030220 oncology & carcinogenesis medicine Hydroxyl radical |
Zdroj: | Journal of Applied Phycology. 30:1641-1653 |
ISSN: | 1573-5176 0921-8971 |
DOI: | 10.1007/s10811-018-1397-2 |
Popis: | The sulfated polysaccharides (SPs) from Chlamydomonas reinhardtii (Cr) were isolated by hot water method using 80% alcohol and semi purified by anion-exchange column chromatography. The chemical analysis of the extract showed 78% carbohydrates, 18% reducing sugars, 60% non-reducing sugars, 2% protein, 33% sulfate, 39% uronic acid, and 4% ash. The elemental analysis of this C. reinhardtii sulfated polysaccharide-enriched extract (Cr-SPs) showed 53% carbon, 8% hydrogen, and 6% nitrogen. FTIR analysis of Cr-SPs showed characteristic bands of sulfated polysaccharides. Further, the Cr-SPs showed significant hydroxyl radical scavenging activity of 22.29–80.9% at 0.01–1 mg mL−1, 38–77% of DPPH radical scavenging activity at 0.01–1 mg mL−1, 9.8–81% ABTS radical scavenging activity, 34.5–67.6% of ferrous chelating ability, and 11.62–75% of total antioxidant capacity. Cr-SPs also showed efficient in vitro anticancer activity. Specifically, they inhibited triple-negative breast cancer cell (MDA-MB-231) proliferation with an IC50 of 172 μg mL−1. Concentration-dependent reduction in the number of colonies formed by MDA-MB-231 cells suggested their potential to inhibit the clonal expansion of the cancer cells. Higher concentrations of crude extract were found to disrupt the microtubule networks in these cells. The cells treated with Cr-SPs eventually underwent apoptosis as evidenced by the formation of characteristic DNA ladder. These results indicate that Cr-SPs find promising opportunities for cancer treatment. |
Databáze: | OpenAIRE |
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