| Popis: |
One (1) anti-Blnslocystis serum from a monkey naturally infected with isolateM12and four (4) hyperimmune sera raised in inbred Balb/c mice against crude antigensof two B/astocystis isolates (e and KI~). one each of E/ltamoeba Ilistolytica (HK9) andGiari/in Jall/blia (7404) were used to react with several homologous and heterologousBlastocystis isolates, E.llistofytica, G. lambfia, EIU/olimax 1/(1/1(1 and Bac-4 (Escherid,iacoli isolated from culture medium of a B. JIO",itlis isolate KPl). All anti.Blastocystissera did not showcross'reactivity with E. Mstolyticn and G.lnlllblin by western blotting.Similarly, anti-E. Iristolyticn and anti-G. lnmblin sera also did not react against allBlnstocystis isolates tested, even though these tmet' protozoa are known to producediarrhoea in humans. Polycional sera raised against antigen prepared from xenic cultureof Blastocystis produced a smear reaction on the immunoblot, while antibodies raisedagainst antigens prepared from axenic culture (isolate q gave prominent reaction bands.This may be due to the purity of the immunogen used in inducing the immune response.The cros~reactionsof sera from mice immunised with the xenic B. IlOlIIitris isolatesmay also due to antibodies against E. coli. Anti-Blastocyst;s serum Crom monkey'swith natural infection showed several prominent reaction bands together with a smearat above 40 kD were most probably induced by the excretory-sKretory antigens of theparasite. A variety of reaction patterns were obtained with these anti'sera and theantigens from different Blnstocystis isolates. These may be reClecis from differencesin antigenic components from various strains of this parasite. |
