FRACTIONATION OF NEUTRAL URINARY STEROIDS1
| Autor: | William H. Pearlman, Gregory Pincus |
|---|---|
| Rok vydání: | 1941 |
| Předmět: | |
| Zdroj: | Endocrinology. 29:413-424 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-29-3-413 |
| Popis: | AN EXTENSIVE LITERATURE dealing with the measurement of urinary 17-ketosteroids has accumulated in the past few years. The determinations are ordinarily based upon the color reaction of ketones with m-dinitrobenzene originally described by Zimmerman (1,2). It has been shown that the 17-ketosteroids give a characteristic color with m-dinitrobensene (2–5). But according to Holtorffand Koch (6), acetone and other ketones also give the same color. Steroids with carbonyl groups in positions other than the 17-position give a low color titer. It has indeed been generally recognized that urines contain materials either non-ketonic or non-steroidal that add to the color titer making it “too high” and in many cases lackingany semblance of agreement with biological activity. Several methods have been employed to bring this titer to rational levels. On the assumption that chromogenic pigments are largely responsible for the overestimate various adsorption methods have been resorted to for removing pigment from the ne... |
| Databáze: | OpenAIRE |
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