Is low-energy-ion bombardment generated X-ray emission a secondary mutational source to ion-beam-induced genetic mutation?
| Autor: | L.D. Yu, D. Suwannakachorn, P. Thopan, K. Prakrajang, P. Thongkumkoon, C. Yaopromsiri |
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| Rok vydání: | 2013 |
| Předmět: |
In situ
Nuclear and High Energy Physics Argon Ion beam Chemistry Astrophysics::High Energy Astrophysical Phenomena X-ray Analytical chemistry chemistry.chemical_element medicine.disease_cause Ion bombardment Metal visual_art Mutation (genetic algorithm) visual_art.visual_art_medium medicine Physics::Accelerator Physics Instrumentation Escherichia coli |
| Zdroj: | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 307:592-597 |
| ISSN: | 0168-583X |
| DOI: | 10.1016/j.nimb.2012.11.066 |
| Popis: | Low-energy ion beam biotechnology has achieved tremendous successes in inducing crop mutation and gene transfer. However, mechanisms involved in the related processes are not yet well understood. In ion-beam-induced mutation, ion-bombardment-produced X-ray has been proposed to be one of the secondary mutation sources, but the speculation has not yet been experimentally tested. We carried out this investigation to test whether the low-energy ion-beam-produced X-ray was a source of ion-beam-induced mutation. In the investigation, X-ray emission from 29-keV nitrogen- or argon- ion beam bombarded bacterial Escherichia coli ( E. coli ) cells held in a metal or plastic sample holder was in situ detected using a highly sensitive X-ray detector. The ion beam bombarded bacterial cells held in different material holders were observed for mutation induction. The results led to a conclusion that secondary X-ray emitted from ion-beam-bombarded biological living materials themselves was not a, or at least a negligible, mutational source, but the ion-beam-induced X-ray emission from the metal that made the sample holder could be a source of mutation. |
| Databáze: | OpenAIRE |
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