Correlation Between Defects in Chromatin Condensation of Human Spermatozoa Stained by Aniline Blue and Semen Characteristics

Autor:	M.L. Guihard‐Moscato, Marie-Jeanne Mayaux, J P Dadoune
Rok vydání:	2009
Předmět:	education.field_of_study medicine.diagnostic_test Sperm chromatin condensation Chemistry Urology Population Semen General Medicine Semen analysis Sperm Staining Chromatin Andrology Endocrinology Prophase medicine education
Zdroj:	Andrologia. 20:211-217
ISSN:	1439-0272 0303-4569
Popis:	Human sperm heads which present disturbances of chromatin condensation are stained by acidic aniline, blue. To determine whether the proportion of unstained heads, i.e. with well condensed chromatin, can be considered as an index of sperm quality, a study was undertaken in 157 men during an infertility evaluation. In addition to the usual sperm characteristics, the percentages of unstained heads and of morphologically normal and abnormal forms were concomitantly evaluated. In a total of 15760 spermatozoa, the percentage of unstained heads was much higher in the population of morphologically normal forms than in that of abnormal forms (79.1% and 49.4% respectively, p less than 10(-9]. Among spermatozoa with structural abnormalities, it was much higher in cells with a single anomaly than in those with associated anomalies (53.9% and 40.6% respectively, p less than 10(-9]. When morphology was taken into account, only vitality was found to vary significantly with the percentage of unstained heads.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::59bb54925257a99be3fa1046ba14e58c https://doi.org/10.1111/j.1439-0272.1988.tb01058.x Zobrazit plný text záznamu