Apoptosis effect of Sinularia leptoclados, S. depressan and S. inflate extracts in human oral squamous cell carcinomas

Autor:	Da-Long Cheng, Tzung-Han Chou, Guey-Horng Wang, Wei Jing Hung, Chia-Hua Liang, Rong-Jyh Lin
Rok vydání:	2010
Předmět:	medicine.diagnostic_test General Chemical Engineering Cell Spheroid General Chemistry Biology biology.organism_classification Molecular biology Flow cytometry Cell biology medicine.anatomical_structure Prophase Apoptosis medicine Viability assay Sinularia Cell adhesion
Zdroj:	Journal of the Taiwan Institute of Chemical Engineers. 41:86-91
ISSN:	1876-1070
DOI:	10.1016/j.jtice.2009.05.014
Popis:	This work demonstrates the toxic effects of Sinularia leptoclados, Sinularia depressan and Sinularia inflate extracts on human oral squamous cell carcinoma (SCC4, SCC9 and SCC25) cells using cell adhesion and cell viability assays. The three extracts inhibited growth of human SCC25 (which exhibited effective differentiation) cells than they did SCC4 (moderate differentiation) and SCC9 (poor differentiation) cells. The morphological changes of the SCC25 cells upon treatment with the three extracts were caused by apoptosis, as determined from the morphological features (spheroid shape, chromatin condensation and formation of apoptotic bodies). Flow cytometry indicates that three extracts sensitized SCC25 cells in the G2/M phases with a concomitantly and significantly increased fraction of sub-G1. This investigation reveals that apoptosis that occurred when the SCC cells were treated with S. leptoclados, S. depressan and S. inflate extracts was accompanied by up-regulation of p53 and activation of caspase-3 expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::59b58b3bb68015d6eb2f2fc53a4bf5b5 https://doi.org/10.1016/j.jtice.2009.05.014 Zobrazit plný text záznamu Full Text from ScienceDirect