Preparation of Retinas for Studying Photoreceptors with Confocal Microscopy
| Autor: | Brian Matsumoto, Irene L. Hale |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Retina
Materials science genetic structures Nanotechnology Retinal Fluorescence eye diseases law.invention chemistry.chemical_compound medicine.anatomical_structure Diverse population chemistry Confocal microscopy law Confocal laser scanning microscopy medicine Biophysics sense organs Cytoskeleton Integral membrane protein |
| DOI: | 10.1016/b978-0-12-185279-5.50009-1 |
| Popis: | Publisher Summary Laser scanning confocal microscopy (LSCM) is an alternate method available for obtaining the optical equivalent of thin fluorescent sections. The optical principle of confocal microscopy is simple, but a large investment in hardware and software is required. LSCM optically isolates fluorescence emission to the image plane, providing a means of obtaining high-resolution images of photoreceptor cells in thick sections. This chapter describes the procedures and techniques for preparing retinas for confocal microscopy. The chapter also provides a description of the protocol and a detailed paradigm for each procedure. A variety of proteins has been successfully stained with these techniques, including cytoskeletal proteins, integral membrane proteins, and soluble cytoplasmic proteins. In addition, the basic procedure has been applied with lectins as well as fluorescent phalloidins and phallotoxins. The ability to stain a diverse population of retinal proteins with a variety of fluorescent probes demonstrates the general applicability of this technique for localizing any protein within the retina. |
| Databáze: | OpenAIRE |
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