Analysis of intracutaneous inflammatory lesions with skin blisters
| Autor: | Maija Horsmanheimo, Eeva von Willebrand, U. Kiistala, Pekka Häyry |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Pathology
medicine.medical_specialty Cell type integumentary system business.industry Lymphoblast medicine.medical_treatment Immunology Tuberculin Blisters Inflammation bacterial infections and mycoses Subclass Pathology and Forensic Medicine medicine Immunology and Allergy Distribution (pharmacology) medicine.symptom skin and connective tissue diseases business Saline |
| Zdroj: | Clinical Immunology and Immunopathology. 11:445-457 |
| ISSN: | 0090-1229 |
| DOI: | 10.1016/0090-1229(78)90172-1 |
| Popis: | We describe a method enabling quantitative isolation of infiltrating cells from inflammatory skin lesions. The method is based on the generation of small blisters onto the skin. The inflammatory cells accumulate into these blisters and they can be recovered without enzymatic treatment simply with a syringe and a hypodermic needle. In this communication we have used purified protein derivative of tuberculin (PPD)-induced inflammation as a model and analyzed the rate and range of accumulation of inflammatory cells into blisters localizing in the vicinity of the PPD injection site. The reactivity to PPD of the test individual had no significant effect on the size of the blisters. Up to 7 to 10 times more cells were recovered from blisters located over the PPD injection site of strongly tuberculin-positive individuals than from control blisters receiving only saline or from PPD blisters of tuberculin-negative individuals. T lymphocytes were the most prominent single cell type in PPD blisters, followed by monocytes/macrophages, B lymphocytes, plasmablasts/plasmacells, and T lymphoblasts. Granulocytes were infrequent. There was no difference in the blister contents when PPD was injected into the blister, intradermally right under the blister bottom, or subcutaneously 7 to 8 mm deep. This demonstrates that the range of accumulation of inflammatory cells covers the dimensions of skin and suggests that the blister method may also be used to investigate infiltrating cells in different dermatological diseases. |
| Databáze: | OpenAIRE |
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