The analysis of thermophilic microorganismsâ affection to the destruction of oil and its products
| Jazyk: | ruština |
|---|---|
| Rok vydání: | 2022 |
| Předmět: | |
| DOI: | 10.18720/spbpu/3/2023/vr/vr23-906 |
| Popis: | Тема вÑпÑÑкной квалиÑикаÑионной ÑабоÑÑ: «ÐÑенка влиÑÐ½Ð¸Ñ ÑеÑмоÑилÑнÑÑ Ð¼Ð¸ÐºÑооÑганизмов на деÑÑÑÑкÑÐ¸Ñ Ð½ÐµÑÑи и ÐµÑ Ð¿ÑоизводнÑÑ Â»ÐоÑколÑÐºÑ Ð² наÑей ÑÑÑане поÑвÑ, ÑегÑлÑÑно загÑÑзнÑемÑе неÑÑÑÑ, в оÑновном, ÑаÑÐ¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ñ Ð² ÑмеÑеннÑÑ Ð¸ вÑÑÐ¾ÐºÐ¸Ñ ÑиÑоÑÐ°Ñ , акÑÑалÑной задаÑей ÑвлÑеÑÑÑ ÑазÑабоÑка биопÑепаÑаÑов, ÑÑÑекÑивно ÑÑÑÑанÑÑÑÐ¸Ñ Ð¿Ð¾ÑледÑÑÐ²Ð¸Ñ ÑÐ°ÐºÐ¸Ñ Ð·Ð°Ð³ÑÑзнений пÑи невÑÑÐ¾ÐºÐ¸Ñ ÑемпеÑаÑÑÑÐ°Ñ Ð¾ÐºÑÑжаÑÑей ÑÑедÑ.ЦелÑÑ ÑабоÑÑ ÑÑали вÑÑвление и ÑÑавниÑелÑнÑй анализ ÑеÑмоÑилÑнÑÑ Ð±Ð°ÐºÑеÑий-неÑÑедеÑÑÑÑкÑоÑов в пÑÐ¾Ð±Ð°Ñ Ð·Ð°Ð³ÑÑзненнÑÑ Ð¿Ð¾ÑвогÑÑнÑов ÑмеÑеннÑÑ ÑиÑоÑ, а Ñакже опÑеделение ÑÑаммов ÑÑÐ¸Ñ Ð±Ð°ÐºÑеÑий, пеÑÑпекÑивнÑÑ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¿ÑепаÑаÑов, оÑиÑаÑÑÐ¸Ñ Ð½ÐµÑÑÑнÑе загÑÑÐ·Ð½ÐµÐ½Ð¸Ñ Ð² ÑÑловиÑÑ ÑмеÑеннÑÑ Ð¸ ÑевеÑнÑÑ ÑиÑоÑ.Ð ÑабоÑе пÑÑем оÑбоÑа и кÑлÑÑивиÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑобиологиÑеÑÐºÐ¸Ñ Ð¿Ñоб, Ñ Ð¿Ñименением меÑодов поÑледоваÑелÑнÑÑ Ñазведений и вÑÑаÑÐ¸Ð²Ð°Ð½Ð¸Ñ ÑаÑÑений в ÑеÑÑовÑÑ Ð¸ конÑÑолÑнÑÑ Ð³ÑÑÐ¿Ð¿Ð°Ñ , изÑÑили вклад ÑеÑмоÑилÑнÑÑ ÑглеводоÑодокиÑлÑÑÑÐ¸Ñ Ð±Ð°ÐºÑеÑий в деÑÑÑÑкÑÐ¸Ñ Ð½ÐµÑÑи в поÑÐ²Ð°Ñ Ð³. СанкÑ-ÐеÑеÑбÑÑга.Ð ÑезÑлÑÑаÑе бÑли подÑвеÑÐ¶Ð´ÐµÐ½Ñ Ð¿ÑиÑÑÑÑÑвие ÑеÑмоÑилÑнÑÑ Ð±Ð°ÐºÑеÑий во взÑÑÑÑ Ð¿ÑÐ¾Ð±Ð°Ñ Ð¸ Ð¸Ñ ÑоÑÑ Ð½Ð° индивидÑалÑнÑÑ ÑглеводоÑоднÑÑ ÑÑбÑÑÑаÑÐ°Ñ . ÐнеÑение в поÑÐ²Ñ ÑÑамма Ð6 ÑеÑмоÑилÑнÑÑ Ð±Ð°ÐºÑеÑий Ñода Aeribacillus облегÑало вÑживаемоÑÑÑ, ÑоÑÑ Ð¸ ÑазвиÑие ÑаÑÑений в загÑÑзненной неÑÑÑÑ Ð¿Ð¾Ñве. РоÑÑоÑÑимÑлиÑÑÑÑий ÑÑÑекÑ, оказÑваемÑй на ÑаÑÑÐµÐ½Ð¸Ñ Ð²Ð½ÐµÑением в загÑÑзненнÑÑ Ð¿Ð¾ÑÐ²Ñ Ð¼Ð¸ÐºÑооÑганизмов ÑÑамма P6-1 Ñода Parageobacillus, бÑл вÑÑажен ÑÑÑеÑÑвенно Ñлабее. Ð¡Ð´ÐµÐ»Ð°Ð½Ñ Ð²ÑÐ²Ð¾Ð´Ñ Ð¾Ð± ÑÑаÑÑии ÑеÑмоÑилÑнÑÑ ÑглеводоÑодокиÑлÑÑÑÐ¸Ñ Ð±Ð°ÐºÑеÑий в деÑÑÑÑкÑии неÑÑи в конÑÑаÑÑнÑÑ ÐºÐ»Ð¸Ð¼Ð°ÑиÑеÑÐºÐ¸Ñ ÑÑловиÑÑ Ð¸ оÑмеÑена возможноÑÑÑ Ð¸ÑполÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑокаÑÐ¸Ð¾Ñ ÑÑамма Ð6 пÑи ÑазÑабоÑке биопÑепаÑаÑа Ð´Ð»Ñ ÑемедиаÑии и оÑиÑÑки загÑÑзненнÑÑ Ð½ÐµÑÑÑÑ Ð¿Ð¾Ñв. The qualification thesis title: «The analysis of thermophilic microorganismsâ affection to the destruction of oil and its products»Since the oil-polluted soils in our country are mainly located at temperate and high latitude, the development of biopreparations for efficient elimination of oil pollution consequences under low temperature conditions remains the important task.The present study is aimed to the revealing and comparative analysis of thermophilic oil-degrading bacteria in the samples of oil-polluted soil taken at temperate latitude as well as the determination of bacterial strain, which is promising for design of biopreparations for eliminations of oil pollution at temperate and high latitude.The contribution of the thermophilic hydrocarbon-degrading bacteria to the oil destruction in soils of St. Petersburg is studied in present work by soil sampling and cultivation of bacteria, containing in the samples, followed by serial dilutions of analyzing samples and the growing of several test and control groups of plants.Both the thermophilic bacteria occurrence in soil samples and their growth on individual hydrocarbonic substrates were confirmed. The introduction into oil-polluted soil of oil-oxidizing thermophilic bacteria of K6 strain (Aeribacillus gen.) facilitated plant survival, growth and development. The growth-stimulating effect to plants of P6-1 strain (Parageobacillus gen.) introduction into soil was found to be significantly weaker. The conclusions about the thermophilic oil-oxidizing bacteria involvement to the oil destruction, occurring under contrasting climatic conditions are made, and the possibility of using of K6 strain prokaryotes for the development of biopreparations aimed to the polluted soils remediation and purification is noted. |
| Databáze: | OpenAIRE |
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