Critical events leading to cellular mortality of yeast cells following hydrostatic pressure treatment
| Autor: | Ukyo Takahashi, Akihisa Miwa, Hitoshi Iwahashi, Kazuhiro Hamada |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Chemistry Endoplasmic reticulum Hydrostatic pressure Saccharomyces cerevisiae Cell Golgi apparatus 010502 geochemistry & geophysics Condensed Matter Physics biology.organism_classification 01 natural sciences Yeast Cell biology symbols.namesake medicine.anatomical_structure 0103 physical sciences Organelle medicine symbols Nuclear membrane 010306 general physics 0105 earth and related environmental sciences |
| Zdroj: | High Pressure Research. 39:267-272 |
| ISSN: | 1477-2299 0895-7959 |
| DOI: | 10.1080/08957959.2018.1556650 |
| Popis: | Hydrostatic pressures of 40–150 MPa are sub-lethal conditions for yeast cells. On exposure to this pressure range, damaged yeast cells will attempt to recover, but a critical cellular event may ultimately lead to cell mortality. We employed yeast strains whose cellular organelles were labeled with green fluorescent protein to investigate this critical event. We were able to visualize the nuclear membrane, nucleus, endoplasmic reticulum, Golgi body, and plasma membranes. Of the cellular organelles tested, the nuclear membrane was the weakest, displaying damage following only 50 MPa of pressure. This nuclear membrane rupture correlated with cellular viability. Thus, we hypothesize that nuclear membrane damage is the critical event leading to cellular mortality of yeast cells following hydrostatic pressure treatment. |
| Databáze: | OpenAIRE |
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