EZH2 in B lymphocyte commitment (HEM1P.229)
| Autor: | Jennifer Jacobsen, Barbara Kee |
|---|---|
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 194:50.12-50.12 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.194.supp.50.12 |
| Popis: | EZH2 is the histone methyltransferase subunit of the Polycomb Repressive Complex 2 that catalyzes the repressive histone modification, H3K27me3. We hypothesize that EZH2 is required to repress alternative lineages during B cell development. Loss of EZH2 in hematopoietic cells leads to blocks in B and T cell development. Here we use an Il7racre mouse model to delete Ezh2 at the common lymphoid progenitor stage to study the role of EZH2 in B lymphocyte commitment. We confirm that Il7racreEzh2fl/fl mice have a cell-intrinsic block at the pro-B cell stage of development. Although there are normal numbers of pro-B cells in Il7racreEzh2fl/fl mice, EZH2-deficient pro-B cells are outcompeted by WT cells in competitive chimeras. By qPCR we find that there is modest upregulation of Gata3 and Nfil3, which regulate innate lymphocyte development. Future studies will investigate the developmental plasticity of EZH2-deficient pro-B cells. |
| Databáze: | OpenAIRE |
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