| Popis: |
The separation of menaquinones (MKs) and phylloquinone (K1) by high-performance liquid chromatography (HPLC) was investigated using silica, cyanobonded and reversed-phase supports. On silica and cyano-bonded supports it was possible to completely resolve cis-and trans-K1, MK-4 nd MK-10, thogh MKs with intermediate side chain lengths were incompletely resolved from each other. Partially saturated MKs with the same side chain length could be completely separated from each other or from their parent quinones on a column of Spherisorb-5 silica when the difference between them was equal to or greater than two saturated prenyl units. A mixture of K1 and MKs 4–10 which included the saturated forms of MK-8(H4), MK-9(H6 and MK-9(H8) could be resolved by reversed-phase HPLC within 30 min. |
