The effect of Pseudomonas fluorescens biosurfactant pseudofactin II on the conformational changes of bovine serum albumin: Pharmaceutical and biomedical applications
| Autor: | Joanna Gałęzowska, Marcin Łukaszewicz, Tomasz Janek, Żaneta Czyżnikowska |
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| Rok vydání: | 2019 |
| Předmět: |
Circular dichroism
Conformational change Quenching (fluorescence) biology Chemistry Binding energy Isothermal titration calorimetry Pseudomonas fluorescens 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 01 natural sciences Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Electronic Optical and Magnetic Materials Materials Chemistry Biophysics biology.protein Physical and Theoretical Chemistry Bovine serum albumin 0210 nano-technology Spectroscopy |
| Zdroj: | Journal of Molecular Liquids. 288:111001 |
| ISSN: | 0167-7322 |
| Popis: | Herein, we have investigated the binding interaction of bovine serum albumin (BSA) with a biosurfactant pseudofactin II (PF) in physiological buffer medium through the use of surface tension, fluorescence, circular dichroism (CD), isothermal titration calorimetry (ITC) and molecular docking studies. Fluorescence results showed that PF interacts with BSA through a static quenching mechanism. CD results demonstrate a conformational change in BSA upon PF combination. The negative Gibbs free energies obtained through ITC experiment suggest that the binding process is spontaneous. Molecular docking further provides an insight of various residues that are involved in this binding process, showing a binding energy of −58.6 kcal mol−1. The present study will help understand the binding mechanism of lipopeptide biosurfactant (PF) to proteins as well as the associated stability and conformational changes. |
| Databáze: | OpenAIRE |
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