Measurement and reliability issues in resonant mode cantilever for bio-sensing application in fluid medium
| Autor: | Priyanka Bhadra, Enakshi Bhattacharya, G Kathel, Anil Prabhakar, Anju Chadha, M. S. Shajahan |
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| Rok vydání: | 2016 |
| Předmět: |
010302 applied physics
Detection limit Cantilever business.industry Chemistry Mechanical Engineering Analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology Laser 01 natural sciences Signal Electronic Optical and Magnetic Materials Transverse mode law.invention Phase-locked loop Optics Interference (communication) Mechanics of Materials Normal mode law 0103 physical sciences Electrical and Electronic Engineering 0210 nano-technology business |
| Zdroj: | Journal of Micromechanics and Microengineering. 26:095007 |
| ISSN: | 1361-6439 0960-1317 |
| DOI: | 10.1088/0960-1317/26/9/095007 |
| Popis: | Cantilevers immersed in liquid experience viscous damping and hydrodynamic loading. We report on the use of such cantilevers, operating in the dynamic mode with, (i) frequency sweeping and (ii) phase locked loop methods. The solution to reliability issues such as random drift in the resonant peak values, and interference of spurious modes in the resonance frequency spectrum, are explained based on the actuation signal provided and laser spot size. The laser beam spot size and its position on the cantilever were found to have an important role, on the output signal and resonance frequency. We describe a method to distinguish the normal modes from the spurious modes for a cantilever. Uncertainties in the measurements define the lower limit of mass detection (m min). The minimum detection limits of the two measurement methods are investigated by measuring salt adsorption from phosphate buffer solution, as an example, a mass of 14 pg was measured using the 14th transverse mode of a m × 100 μm × 1 μm silicon cantilever. The optimized measurement was used to study the interaction between antibody and antigen. |
| Databáze: | OpenAIRE |
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