| Popis: |
1 We evaluated whether immunization aAects bone-marrow responses to indomethacin, because allergenic sensitization and challenge upregulate responses to haemopoietic cytokines (including IL5-driven eosinopoiesis) in murine bone-marrow, while indomethacin upregulates haemopoiesis and protects bone-marrow from radiation damage. 2 Progenitor (semi-solid) and/or precursor (liquid) cultures were established from bone-marrow of: (a) normal mice; (b) ovalbumin-sensitized mice, with or without intranasal challenge. Cultures were established with GM-CSF (2 ng ml 71 ) or IL-5 (1 ng ml 71 ), respectively, alone or associated with indomethacin (10 77 ‐10 711 M) or aspirin (10 77 ‐10 78 M). Total myeloid colony numbers and numbers of eosinophil-peroxidase-positive cells were determined at day 7. |
