Semi-preparative high-performance liquid chromatographic resolution of brompheniramine enantiomers using β-cyclodextrin in the mobile phase
| Autor: | Andrew D. Cooper, T.M. Jefferies |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Chromatography Resolution (mass spectrometry) Cyclodextrin Organic Chemistry Analytical chemistry Chemical modification General Medicine Brompheniramine Biochemistry High-performance liquid chromatography Analytical Chemistry Chiral column chromatography chemistry Phase (matter) medicine Enantiomer medicine.drug |
| Zdroj: | Journal of Chromatography A. 637:137-143 |
| ISSN: | 0021-9673 |
| Popis: | The semi-preparative reversed-phase HPLC separation of brompheniramine enantiomers using β-cyclodextrin as a chiral mobile phase additive is described. Enantiomers were recovered free from mobile phase components on-line by a novel column switching procedure. On a 250 mm × 10 mm I.D. cyano column, a throughput of 8 mg per hour (0.65 mg per gram column packing per hour) was achieved at product optical purities of 88% and above. This was achieved using two passes through the system. Comparison is made with other reported semi-preparative chiral separations using β-cyclodextrin, and the potential utility of the described method discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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