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A 60-day indoor trial was conducted to study the effect of periphyton supplementation on metabolic and immune responses in tiger shrimp, Penaeus monodon. Periphyton developed over bamboo substrate in outdoor tanks (15 m 2 ) was used as dietary supplement for P. monodon (2.02 � 0.04 g) reared in 1000 L FRP tanks. Graded levels of periphyton were included in shrimp basal diets: 0% (P0), 3% (P3), 6% (P6), 9% (P9) and P0 diet with natural periphyton (NP) over bamboo substrate. At the end of the trial, P6 and NP showed significantly higher (P < 0.01) growth rate, 23.9% and 20%, respectively, compared with control, P0. Comparatively, lower level of metabolic enzymes, such as lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase and alanine aminotransferase, was recorded in treatments P3, P6 and NP compared with control, P0. The periphyton-supplemented group, P3 had significantly higher (P < 0.05) superoxide dismutase (15.83 � 0.96) and catalase activity (15.73 � 0.69) compared to 6.88 � 2.84 and 9.15 � 0.67 unit mg � 1 protein min � 1 , respectively, in P0. Similarly, higher |
